Citrus-derived auraptene stimulates angiogenesis by activating the Erk- and PI3K/Akt/eNOS-dependent signaling pathways in human umbilical vein endothelial cells

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• 作者：Shuaiyu Wang ; Yeo Cho Yoon ; Mi-Jeong Sung ; Jin-Taek Hwang ; Haeng-Jeon Hur ; Hyun-Jin Kim ; Hye Jeong Yang ; Myung-Sunny Kim ; Dae Young Kwon ; Jae-Ho Park
• 关键词：Angiogenesis ; Auraptene ; Human umbilical vein endothelial cell ; Signaling pathway
• 刊名：Journal of Functional Foods
• 出版年：2012
• 出版时间：October, 2012
• 年：2012
• 卷：4
• 期：4
• 页码：899-905
• 全文大小：668 K

文摘

Auraptene is a citrus-derived natural monoterpene that has been shown to exert anti-cancer, anti-bacterial, anti-inflammatory, and antioxidant roles. Since little is known about other biological functions of auraptene, we examined the efficacy of auraptene for stimulating angiogenesis in human umbilical vein endothelial cells (HUVECs). Treatment with low concentrations of auraptene stimulated endothelial cell proliferation, migration, and tube formation. Furthermore, auraptene activated Erk, Akt, and endothelial nitric oxide synthase (eNOS), and increased NO production. Auraptene also partially induced the phosphorylation of VEGFR2. Furthermore, auraptene-induced activation of Erk, Akt, and eNOS were significantly inhibited by the inhibitors PD98059, LY294002, and l-NIO dihydrochloride. These results suggest that auraptene stimulates angiogenesis by regulating the VEGFR2, Erk, and PI3K/Akt-eNOS signaling pathways.