Analysis of abamectin residues in avocados by high-performance liquid chromatography with fluorescence detection

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• 作者：Javier Hern&#xe1 ; ndez-Borges ; Lidia M. Ravelo-P&#xe9 ; rez ; Estrella M. Hern&#xe1 ; ndez-Su&#xe1 ; rez ; Aurelio Carnero ; Miguel Á ; ngel Rodr&#xed ; guez-Delgado
• 关键词：High-performance liquid chromatography ; Fluorescence detection ; Abamectin ; Avocados
• 刊名：Journal of Chromatography A
• 出版年：2007
• 出版时间：21 September 2007
• 年：2007
• 卷：1165
• 期：1-2
• 页码：52-57
• 全文大小：282 K

文摘

In this work an analytical method for the determination of abamectin residues in avocados is developed using high-performance liquid chromatography (HPLC) with fluorescence (FL) detection. A pre-column derivatization with trifluoroacetic anhydride (TFAA) and N-methylimidazole (NMIM) was carried out. The mobile phase consisted of water, methanol and acetonitrile (5:47.5:47.5 v/v/v) and was pumped at a rate of 1 mL/min (isocratic elution). The fluorescence detector was set at an excitation wavelength of 365 nm and an emission wavelength of 470 nm. Homogenized avocado samples were extracted twice with acetonitrile:water 8:2 (v/v) and cleaned using C₁₈ solid-phase extraction (SPE) cartridges. Recovery values were in the range 87–98 % with RSD values lower than 13 % . The limits of detection (LODs) and quantification (LOQs) of the whole method were 0.001 and 0.003 mg/kg, respectively. These values are lower than the maximum residue limit (MRL) established by the European Union (EU) and the Spanish legislation in avocado samples.