Molecular identification of interleukin-2 in the lymphoid tissues of the common brushtail possum, Trichosurus vulpecula

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• 作者：L.J.  ; Young^a ;   ; ^c ; M.L.  ; Cross^b ; J.A.  ; Duckworth^b ; S.  ; Flenady^c ; K.  ; Belov^a ;   ; ^{kathy.belov@sydney.edu.au} ;   ; ^{kbelov@vetsci.usyd.edu.au}
• 关键词：Brushtail possum ; Cytokine ; Interleukin-2 ; Marsupial ; Trichosurus vulpecula
• 刊名：Developmental and Comparative Immunology
• 出版年：2012
• 出版时间：January 2012
• 年：2012
• 卷：36
• 期：1
• 页码：236-240
• 全文大小：805 K

文摘

The common brushtail possum (Trichosurus vulpecula) is an Australian marsupial. Here we describe the identification of possum interleukin-2 in mitogen-stimulated lymph node cells. We used a strategy of Rapid amplification of cDNA ends using probes designed from recently-sequenced marsupial genomes to identify the IL2 gene and then confirmed that IL-2 expression in possum immune tissue occurs in a similar manner to that in their eutherian counterparts. The predictive possum IL-2 peptide showed 28 % and 35 % amino acid sequence homology with the mouse and human IL-2 molecules, respectively, consistent with the divergence found within this cytokine family. Despite this low sequence identity, possum IL-2 still possessed the characteristic hallmarks of mammalian IL-2, such as a predicted signal peptide and conserved family motifs.