Crystallization and preliminary X-ray crystallographic analysis of NADPH: azodicarbonyl/quinone oxidoreductase, a plant ζ-crystallin

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• 作者：Mano ; Jun’ ; ichi ; Yoon ; Hye-Jin ; Asada ; Kozi ; Babiychuk ; Elena ; Inzé ; Dirk ; Mikami ; Bunzo
• 关键词：P1 protein ; Crystallization ; ζ ; -crystallin ; Arabidopsis thaliana ; QOR ; quinone oxidoreductase ; ZCr ; ζ ; -crystallin ; P1p ; Arabidopsis thaliana P1 protein
• 刊名：Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular Enzymology
• 出版年：2000
• 出版时间：July 14, 2000
• 年：2000
• 卷：1480
• 期：1-2
• 页码：374-376
• 全文大小：208 K

文摘

Arabidopsis thaliana P1 protein was crystallized using the hanging drop vapor-diffusion method in 0.1 M piperazine-1,4-bis(2-ethanesulfonic acid) buffer, containing 14 % polyethylene glycol 6000 and 0.2 M magnesium acetate at pH 6.5 and 20°C. The crystals are orthorhombic and belong to the space group P2₁2₁2₁ with unit cell dimensions of a