Histone deacetylation-mediated cellular dedifferentiation in Arabidopsis

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• 作者：Kyounghee Lee^a ; Ok-Sun Park^b ; Su-Jin Jung^b ; Pil Joon Seo^a ; ^b ; ^{pjseo1@jbnu.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：AGL15 ; AGAMOUS-LIKE 15 ; ARR ; ARABIDOPSIS RESPONSE REGULATOR ; BBM ; BABY BOOM ; CIM ; callus-inducing medium ; HDAC ; HISTONE DEACETYLASE ; HDT ; HD-TUINS PROTEIN ; LBD ; LATERAL ORGAN BOUNDARIES DOMAIN ; LEC1 ; LEAFY COTYLEDON 1 ; PcG ; polycomb group ; PRC ; polycomb repressive complex ; SRT1 ; SIRTUIN 1 ; TSA ; trichostatin A
• 刊名：Journal of Plant Physiology
• 出版年：2016
• 出版时间：1 February 2016
• 年：2016
• 卷：191
• 期：Complete
• 页码：95-100
• 全文大小：698 K

文摘

Chromatin structure determines the accessibility of transcriptional regulators to target DNA and contributes to regulation of gene expression. Posttranslational modifications of core histone proteins underlie the reversible changes in chromatin structure. Epigenetic regulation is closely associated with cellular differentiation. Consistently, we found that histone deacetylation is required for callus formation from leaf explants in Arabidopsis . Treatment with trichostatin A (TSA) led to defective callus formation on callus-inducing medium (CIM). Gene expression profiling revealed that a subset of HDAC genes, including HISTONE DEACETYLASE 9 (HDA9), HD-TUINS PROTEIN 1 (HDT1), HDT2, HDT4, and SIRTUIN 1 (SRT1), was significantly up-regulated in calli. In support of this, genetic mutations of HDA9 or HDT1 showed reduced capability of callus formation, probably owing to their roles in regulating auxin and embryonic and meristematic developmental signaling. Taken together, our findings suggest that histone deacetylation is intimately associated with the leaf-to-callus transition, and multiple signaling pathways are controlled by means of histone modification during cellular dedifferentiation.