Isolation, purification and chemical characterization of alkali-extractable polysaccharides from the cell walls of Talaromyces species

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• 作者：A. Prieto ; M. Bernabé ; J.A. Leal
• 刊名：Mycological Research
• 出版年：1995
• 出版时间：January 1995
• 年：1995
• 卷：99
• 期：1
• 页码：69-75
• 全文大小：914 K

文摘

From the alkali-extractable material from cell walls of some species of Talaromyces a water-extractable (F1S) and a water-unextractable (F1I) fraction were obtained. Fraction F1S (representing 2·5–12·5 % of the cell wall) was filtered through Sepharose CL-6B in order to purify polysaccharides that were individually studied for chemical composition and structure. According to the structure of these polysaccharides, the species investigated could be grouped as follows: T. stipitatus, T. bacillisporus and T. macrosporus had as their main component a heteropolysaccharide containing β-(1 → 2) and β-(1 → 5) galactofuranosyl residues; in T. wortmannii and T. rotundus the most abundant component was a polysaccharide with β-(1 → 2) and β-(1 → 3)-linked galactofuranose; T. luteus contained in fraction F1S a single polysaccharide, identified as a highly branched galacto-gluco-mannan. Fraction F1I was identified as an α-glucan in most of the species. These results reflect the complexity of the genus and could have chemotaxonomic value.