Selective colorimetric detection of Gram-negative re-contaminants in pasteurised milk products by a novel application of the BacT/ALERT 3D system

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• 作者：Konrad J. Domig^a ; ^{konrad.domig@boku.ac.at} ; Ulrike Zitz^a ; Sonja Macher^a ; Alois Kronberger^b ; Andreas Reiter^b ; Wolfgang Kneifel^a
• 刊名：International Dairy Journal
• 出版年：2013
• 出版时间：March, 2013
• 年：2013
• 卷：29
• 期：1
• 页码：21-27
• 全文大小：914 K

文摘

The BacT/ALERT 3D equipment was applied as a fast and sensitive method for the detection of Gram-negative re-contaminants in pasteurised dairy products, particularly drinking milk and cream. The iAST medium was modified and then applied to eleven certified reference strains and nine relevant wild-type isolates originating from a dairy plant. Gram-negative strains could be detected at low levels in pure culture and in combination with high numbers of Gram-positive bacteria as background microflora. Additional experiments with qualified reference material (Escherichia coli, Pseudomonas aeruginosa) showed that low levels of contamination of these bacteria were detectable within 1 and 1.5 days, respectively. The milk matrices exerted no relevant effect on the detectability of the Gram-negative re-contaminants tested. Based on the technique developed, a faster hygiene monitoring and enhanced approval of dairy product lots at the production site is enabled.