Raman and SERS Spectroscopy of D96N Mutant Bacteriorhodopsin

详细信息    查看全文

• 作者：Konstantin Mochalov^a ; ^b ; Daria Solovyeva^a ; ^b ; Anton Chistyakov^a ; ^b ; Boris Zimka^b ; Eugeni Lukashev^c ; Igor Nabiev^a ; ^d ; Vladimir Oleinikov^a ; ^b ; ^{voleinik@mail.ru" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Bacteriorhodopsin ; mutant D96N ; silver nanoparticles ; puple membrane ; Raman scattering ; SERS
• 刊名：Materials Today: Proceedings
• 出版年：2016
• 出版时间：2016
• 年：2016
• 卷：3
• 期：2
• 页码：497-501
• 全文大小：160 K

文摘

Effect of silver nanoparticles (AgNPs) on the D96N mutant form of bacteriorhodopsin (BRh-D96N) contained in fragments of purple membranes (PMs) of bacteria Halobacterium salinarum has been analyzed. It has been found that interaction of AgNPs with BRh-D96N in SERS-active spots leads to “freezing” of the protein photocycle. The AgNPs change and then fix the state of BRh-D96N in SERS-active spots. Outside of SERS-active spots, the effect of AgNPs is weaker and is expressed in deceleration of the M-form decay. Thus, the fundamental difference in the influence of AgNPs on the mutant BRh-D96N in comparison with wild-type BRh was found.