Ethnic diversity of gut microbiota: Species characterization of Bacteroides fragilis group and genus Bifidobacterium in healthy Belgian adults, and comparison with data from Japanese subjects

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• 作者：Eiji Ishikawa¹ ; ² ; ^{eiji-ishikawa@yakult.co.jp} ; Takahiro Matsuki¹ ; Hiroyuki Kubota² ; Hiroshi Makino² ; Takafumi Sakai² ; Kenji Oishi² ; Akira Kushiro¹ ; ² ; Junji Fujimoto¹ ; Koichi Watanabe¹ ; Masaaki Watanuki¹ ; ² ; Ryuichiro Tanaka¹ ; ²
• 关键词：Bifidobacterium catenulatum group ; Dietary habit ; Quantitative PCR ; Microbiota structure ; Species-level
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2013
• 出版时间：August, 2013
• 年：2013
• 卷：116
• 期：2
• 页码：265-270
• 全文大小：1128 K

文摘

The composition of the human gut microbiota is related to host health, and it is thought that dietary habits may play a role in shaping this composition. Here, we examined the population size and prevalence of six predominant bacterial genera and the species compositions of genus Bifidobacterium (g-Bifid) and Bacteroides fragilis group (g-Bfra) in 42 healthy Belgian adults by quantitative PCR (qPCR) over a period of one month. The population sizes and prevalence of these bacteria were basically stable throughout the study period. The predominant g-Bifid species were Bifidobacterium adolescentis and Bifidobacterium longum ss. longum, and the predominant g-Bfra species were Bacteroides vulgatus, Bacteroides uniformis, and Bacteroides ovatus. The Belgian gut microbiota data were then compared with gut microbiota data from 46 Japanese subjects collected according to the same protocol (Matsuki et?al., Appl. Environ. Microbiol. 70, 167-173, 2004). The population size and prevalence of Bifidobacterium catenulatum group were significantly lower in the Belgian gut microbiota than in the Japanese gut microbiota (P?<?0.001); however, the population size and prevalence of g-Bifid did not differ. This species-level qPCR analysis will be helpful for investigating the diversity of gut microbiota among ethnic groups.