Generation of virus like particles for epizootic hemorrhagic disease virus
详细信息 查看全文
- 作者：Mario Forzana ; ; Sushila Maanb ; Maurizio Mazzeia ; Manjunatha N. Belaganahallib ; Lucia Bonuccellia ; Monica Calamaric ; Maria Luisa Carrozzad ; Valentina Cappelloe ; Mariagrazia Di Lucaf ; Patrizia Bandecchia ; Peter P.C. Mertensb ; Francesco Tolaria
- 关键词：Epizootic hemorrhagic disease virus ; Baculovirus expression system ; Insect cells ; Virus like particles
- 刊名：Research in Veterinary Science
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：107
- 期：Complete
- 页码：116-122
- 全文大小：888 K
文摘
- •
We cloned the structural proteins of Epizootic Hemorrhagic Disease Virus serotype 6.
- •
Two recombinant baculoviruses each expressing two viral structural proteins were generated.
- •
The presence of mRNAs and respective viral proteins was confirmed by infection of insect cells.
- •
Virus like particles were purified and visualized by Transmission Electron Microscope.