Detection of antibiotic residues and association of cefquinome residues with the occurrence of Extended-Spectrum 尾-Lactamase (ESBL)-producing bacteria in waste milk samples from dairy farms in England and Wales in 2011

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• 作者：Luke R ; all ; Katharina Heinrich ; Robert Horton ; Lucy Brunton ; Matthew Sharman ; Victoria Bailey-Horne ; Meenaxi Sharma ; Ian McLaren ; Nick Coldham ; Chris Teale ; Jeff Jones
• 关键词：Cephalosporin ; Resistance ; CTX-M ; blaCTX-M ; Waste milk
• 刊名：Research in Veterinary Science
• 出版年：February, 2014
• 年：2014
• 卷：96
• 期：1
• 页码：15-24
• 全文大小：937 K

文摘

Waste milk samples from 103 farms in England and Wales were examined for the presence of 尾-lactam antibiotics and ESBL-producing Enterobacteriaceae. Approximately 10 months after the initial sampling, further waste milk, environmental and faecal samples from farms shown to be positive for CTX-M Escherichia coli were investigated further. Isolates with an ESBL phenotype were tested by PCR for the presence of bla_CTX-M, bla_OXA, bla_SHV and bla_TEM genes. Isolates positive for bla_CTX-M were sequenced to determine CTX-M type. Representative isolates were further examined by PFGE, plasmid replicon typing and serotyping. Of particular interest, 21.4% of waste milk samples contained residues of the cephalosporin cefquinome, which was significantly associated with CTX-M bacteria. Such bacteria occurred in 5.8% of the waste milk samples (including 3.9% CTX-M E. coli). CTX-M types identified were 1, 14, 14b and 15, but none of the E. coli were serotype O25, the serotype of the human pandemic strain.