Efficient removal of lignin with the maintenance of hemicellulose from kenaf by two-stage pretreatment process
详细信息 查看全文
- 作者：Nur Izyan Wan Azelee ; Jamaliah Md Jahim ; Amir Rabu ; Abdul Munir Abdul Murad ; Farah Diba Abu Bakar ; Rosli Md Illias
- 关键词：Hemicellulose ; Kenaf ; Lignin removal ; Pretreatment
- 刊名：Carbohydrate Polymers
- 出版年：2 January, 2014
- 年：2014
- 卷：99
- 期：Complete
- 页码：447-453
- 全文大小：1170 K
文摘
The enhancement of lignocellulose hydrolysis using enzyme complexes requires an efficient pretreatment process to obtain susceptible conditions for the enzyme attack. This study focuses on removing a major part of the lignin layer from kenaf (Hibiscus cannabinus) while simultaneously maintaining most of the hemicellulose. A two-stage pretreatment process is adopted using calcium hydroxide, Ca(OH)2, and peracetic acid, PPA, to break the recalcitrant lignin layer from other structural polysaccharides. An experimental screening of several pretreatment chemicals, concentrations, temperatures and solid-liquid ratios enabled the production of an optimally designed pretreatment process for kenaf. Our results showed that the pretreatment process has provide 59.25% lignin removal while maintaining 87.72% and 96.17% hemicellulose and cellulose, respectively, using 1 g of Ca(OH)2/L and a 8:1 (mL:g) ratio of liquid-Ca(OH)2 at 50 掳C for 1.5 h followed by 20% peracetic acid pretreatment at 75 掳C for 2 h. These results validate this mild approach for aiding future enzymatic hydrolysis.