A multiplex PCR assay for the direct identification of the capsular type (Ia to IX) of Streptococcus agalactiae
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- 作者：Monica Imperi ; Marco Pataracchia ; Giovanna Alfarone ; Lucilla Baldassarri ; Graziella Orefici ; Roberta Creti
- 关键词：Group B streptococcus ; S. agalactiae ; Multiplex PCR ; Capsular operon ; Serotype
- 刊名：Journal of Microbiological Methods
- 出版年：2010
- 出版时间：February 2010
- 年：2010
- 卷：80
- 期：2
- 页码：212-214
- 全文大小：203 K
文摘
A multiplex PCR assay for the identification of serotypes Ia to IX of Streptococcus agalactiae was developed. By using a single PCR reaction containing a mix of 19 primers the assay identified each serotype by the analysis of the unique two or three bands pattern on agarose gel.