Small-angle neutron scattering study of the n-decane effect on the bilayer thickness in extruded unilamellar dioleoylphosphatidylcholine liposomes

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• 作者：Uhrí ; ková ; Daniela ; Balgav&yacute ; Pavol ; Kucerka ; Norbert ; Islamov ; Akhmed ; et. al.
• 关键词：1 ; 2-Dioleoyl-3-phosphatidylcholine ; Unilamellar liposomes ; n-Decane ; Bilayer thickness ; Small-angle neutron scattering
• 刊名：Biophysical Chemistry
• 出版年：2000
• 出版时间：December 15, 2000
• 年：2000
• 卷：88
• 期：1-3
• 页码：165-170
• 全文大小：116 K

文摘

Dioleoylphosphatidylcholine (DOPC) and n-decane were mixed and hydrated afterwards in an excess of heavy water at 1 wt. % of DOPC. From this dispersion, unilamellar liposomes were prepared by extrusion through polycarbonate filter with 500-Å pores. Small-angle neutron scattering (SANS) was conducted on these liposomes. From the Kratky–Porod plot ln[I(Q)Qp>2p>] vs. Qp>2p> of SANS intensity I(Q) in the range of scattering vectors Q corresponding to the interval 0.001 Åp>−2p>≤Qp>2p>≤0.006 Åp>−2p>, the liposome bilayer radius of gyration R_g and the bilayer thickness parameter d_g