Development and validation of isomer specific RP-HPLC method for quantification of cefpodoxime proxetil
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- 作者：Vasu Kumar Kakumanu ; Vinod Kumar Arora and Arvind Kumar Bansal
- 关键词：Racemic cefpodoxime proxetil ; R ; S-enantiomers ; Separation of isomers ; Reversed-phase ; HPLC ; Liquid chromatography
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2006
- 出版时间：1 May 2006
- 年：2006
- 卷：835
- 期：1-2
- 页码：16-20
- 全文大小：173 K
文摘
The present work explains the development and validation of a simple and reliable isomer specific liquid chromatographic method for the quantitative determination of cefpodoxime proxetil (CP) in rat in situ intestinal perfusate samples. Chromatography was carried out by reversed-phase technique on a C-18 column with a mobile phase composed of 20 mM ammonium acetate buffer (pH 5.0) and acetonitrile in the ratio of 62:38 pumped at a flow-rate of 1 ml/min. The detection was carried out at 235 nm and a column temperature of 30 °C. The method was evaluated for the various validation parameters, such as linearity, accuracy, precision, LOD, LOQ, specificity, selectivity, and sample stability. The results of intra- and inter-day validation (n = 3) showed the method to be efficient and the same was applied in an in situ permeability study conducted for CP in rats.