Kinase activity of OsBRI1 is essential for brassinosteroids to regulate rice growth and development

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• 作者：Jinfeng Zhao^a ; Chenxi Wu^b ; Shoujiang Yuan^c ; Liang Yin^c ; Wei Sun^c ; Qinglei Zhao^c ; Baohua Zhao^b ; Xueyong Li^a ; ^{xueyong.li@caas.net.cn}
• 关键词：BRASSINOSTEROID INSENSITIVE 1 (BRI1) ; DWARF 61 (D61) ; dwarf54 (d54) ; Kinase activity
• 刊名：Plant Science
• 出版年：2013
• 出版时间：February, 2013
• 年：2013
• 卷：199-200
• 期：Complete
• 页码：113-120
• 全文大小：1219 K

文摘

Brassinosteroids (BRs) are steroid hormones that participate in multiple biological processes. In this paper, we characterized a classic rice mutant Fn189 (dwarf54, d54) showing semi-dwarf stature and erect leaves. The coleoptile elongation and root growth was less affected in Fn189 than wild-type plant by the exogenous application of eBL, the most active form of BRs. Lamina joint inclination assay and morphological analysis in darkness further showed that Fn189 mutant plant was insensitive to exogenous eBL. Through map-based cloning, Fn189 was found to be a novel allelic mutant of the DWARF 61 (D61) gene, which encodes the putative BRs receptor OsBRI1. A single base mutation caused the I834F substitution in the OsBRI1 kinase domain. Consequently, kinase activity of OsBRI1 was found to decrease dramatically. Taken together, the kinase activity of OsBRI1 is essential for brassinosteroids to regulate normal plant growth and development in rice.