Interaction of anticancer herbal drug berberine with DNA immobilized on the glassy carbon electrode

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• 作者：Xue Tian ; Yaheng Song ; Huimin Dong ; Baoxian Ye
• 关键词：Berberine ; DNA ; Anticancer herbal drug ; Hill parameters ; Langmuir– ; Blodgett film
• 刊名：Bioelectrochemistry
• 出版年：2008
• 出版时间：June 2008
• 年：2008
• 卷：73
• 期：1
• 页码：18-22
• 全文大小：628 K

文摘

The interaction of anticancer herbal drug berberine with double-strand DNA (dsDNA) and single-strand DNA (ssDNA) in solution, dsDNA immobilized on the glassy carbon electrode prepared by Langmuir–Blodgett technique, were investigated by electrochemical techniques (cyclic voltammetry, differential pulse voltammetry) and UV spectroscopy. The presence of DNA results in a decrease of the currents and a negative shift of the electrode potentials from the DPV curves of berberine, indicating the dominance of electrostatic interactions. The spectroscopy data confirmed that the predominant interaction between berberine and DNA is electrostatic. The binding of berberine with DNA, when analyzed in terms of the cooperative Hill model, yields the binding constant K_a = 2.2(± 0.2) × 10⁴ M^{− 1}, corresponding to the dissociation equilibrium constant K_d = 4.6(± 0.3) × 10^{− 5} M, which in the range of the applied concentrations of DNA (bp) and berberine, and a Hill coefficient m = 1.82(± 0.08) in Britton–Robinson buffer solution (0.05 M, pH 5.72) at T = 298 K (25 °C). Apparently, at least two molecules of berberine have to bind as a couple to cause, e.g., the “elementary event” of current change. The results are suggestive for further fruitful applications of this anticancer herbal drug and DNA-modified electrodes.