A comparative study of serological tests for use in the bovine Herpesvirus 1 eradication programme in The Netherlands

详细信息    查看全文

• 作者：de Wit ; J.J. ; Hage ; J.J. ; Brinkhof ; J. ; Westenbrink ; F.
• 关键词：Bovine herpesvirus 1 (BHV1) ; Danish test system ; ELISA ; gB ; gE
• 刊名：Veterinary Microbiology
• 出版年：1998
• 出版时间：March 31, 1998
• 年：1998
• 卷：61
• 期：3
• 页码：153-163
• 全文大小：183 K

文摘

We compared a gB-ELISA, a gE-ELISA and a Danish test system (consisting of a blocking and an indirect ELISA) for their specificity and sensitivity to detect antibodies against BHV1. The Danish test system showed the highest sensitivity and the gE-ELISA the lowest; the gB-ELISA showed an intermediate sensitivity. If the doubtful zone (25–50 % blocking) of the gB-ELISA was considered as positive (gB-ELISA⁺), the sensitivity almost reached that of the Danish test system. The specificity of all tests appeared to be very high, 99.7, 96.7 100, 99.7 % for the gB-ELISA, gB-ELISA⁺, gE-ELISA and the Danish test system, respectively. Seroconversion was detected in the gE-ELISA up to 3 weeks later than in the gB-ELISA and the Danish test system. It is concluded that the combination of a gB-ELISA (for screening) and the Danish test (for confirmation) system used in the BHV1 eradication programme in the Netherlands, provides for very high sensitivity (>99.0 % ) () and a very high specificity (>99.9 % ). Publisher: Elsevier Science Language of Publication: English Item Identifier: S0378-1135(98)00166-7 Publication Type: Article ISSN: 0378-1135 Cited by:

1. Lemaire, Mylene; Schynts, Frederic; Meyer, Gilles; Georgin, Jean-Pierre; Baranowski, Eric; Gabriel, Annick; Ros, Carlos; Belak, Sandor; Thiry, Etienne,""Latency and reactivation of a glycoprotein E negative bovine herpesvirus type 1 vaccine: influence of virus load and effect of specific maternal antibodies""Vaccine2001pp. 4795-4804
   Bibliographic PageFull Text
2. Grisaru, Marcus T.; Knutt, Marcia E.,""Norcor vs the abominable gauge completion¹""Physics Letters B2001pp. 188-198
   Bibliographic PageFull Text
3. Sugiyama, Katsuyuki; Yoshida, Kentaroh,""Supermembrane on the pp-wave background""Nuclear Physics B2002pp. 113-127
   Bibliographic PageFull Text
4. Hyun, Seungjoon; Shin, Hyeonjoon,""Branes from matrix theory in pp-wave background""Physics Letters B2002pp. 115-120
   Bibliographic PageFull Text
5. Vonk Noordegraaf,, A.; Nielen, M.; Franken, P.; Dijkhuizen, A.A.,""Simulation modelling of BHV1-control programme at national level, with special attention to sensitivity analysis""Livestock Production Science2002pp. 153-170
   Bibliographic PageFull Text
6. Metsaev, R.R.,""Type IIB Green-Schwarz superstring in plane wave Ramond-Ramond background""Nuclear Physics B2002pp. 70-96
   Bibliographic PageFull Text
7. Metsaev, R.R.,""Supersymmetric D3 brane and N=4 SYM actions in plane wave backgrounds""Nuclear Physics B2003pp. 3-56
   Bibliographic PageFull Text
8. Hyun, Seungjoon; Shin, Hyeonjoon,""Solvable N=(4,4) type IIA string theory in plane-wave background and D-branes""Nuclear Physics B2003pp. 114-134
   Bibliographic PageFull Text
9. Hyun, Seungjoon; Park, Jaemo; Shin, Hyeonjoon,""Covariant description of D-branes in IIA plane-wave background""Physics Letters B2003pp. 80-88
   Bibliographic PageFull Text
10. Sakaguchi, Makoto; Yoshida, Kentaroh,""Dirichlet branes of the covariant open supermembrane on a pp-wave background""Nuclear Physics B2004pp. 311-324
    Bibliographic PageFull Text

---

Footnotes:

1. Supported in part by National Science Foundation Grant PHY-00-70475.