Depuration dynamics of oysters (Crassostrea gigas) artificially contaminated by Salmonella enterica serovar Typhimurium
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- 作者：Adriana de Abreu Corrê ; a ; Jonas Dutra Albarnaz ; Vanessa Moresco ; Carlos Rogé ; rio Poli ; Adriano Luiz Teixeira ; Clá ; udia Maria Oliveira Simõ ; es ; Cé ; lia Regina Monte Barardi
- 关键词：Oysters Crassostrea gigas ; Depuration ; Salmonella enterica serovar Typhimurium ; PCR
- 刊名：Marine Environmental Research
- 出版年：2007
- 出版时间：June 2007
- 年：2007
- 卷：63
- 期：5
- 页码：479-489
- 全文大小：141 K
文摘
The State of Santa Catarina produces the greatest quantity of edible mollusks in Brazil. To guarantee sanitary qualify, mollusk cultures should be monitored for contamination by pathogenic microorganisms. A self-purification or “depuration” system that eliminates Salmonella enterica serovar Typhimurium contamination from oysters has been developed and evaluated. The depuration process occurred within a closed system, in which 1000 L of water was recirculated for 24 h. The water was sterilized with ultraviolet (UV) light, chlorine, or both together. Oysters (Crassostrea gigas) artificially contaminated with S. typhimurium were harvested every 6 h. Samples of oyster tissue were excised and both the presence and numbers of bacteria were determined. Combined UV light and chlorine treatments resulted in total elimination of bacteria within 12 h. Polymerase chain reaction detected bacteria in water exposed to the three treatments. This pioneering study is the first of its kind in Brazil and represents a major contribution to commercial mollusk culture in this country.