Optimization of the preparation of pectin from Aloe using a Box-Behnken design
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- 作者：Lijing Geng ; gengli777@126.com" class="auth_mail ; Wei Zhou ; Xingyuan Qu ; Weijie Chen ; Yingchang Li ; Chang Liu ; Jing Sun ; Xiaolei Yu ; Hui Wang ; Zhen Zhang ; Jing Li ; Lijie Wang
- 关键词：Aloe vera barbadensis Miller ; High-methoxyl pectin ; Extraction ; Storage ; Viscosity
- 刊名：Carbohydrate Polymers
- 出版年：25 May, 2014
- 年：2014
- 卷：105
- 期：Complete
- 页码：193-199
- 全文大小：1819 K
文摘
The extraction condition of pectin from Aloe vera barbadensis Mill was optimized by a Box-Behnken design. The effect of parameters of extraction water proportion (EWP), extraction pH (EpH), extraction temperature (ETe), extraction time (ETi), alcohol precipitation pH (APpH)and alcohol precipitation temperature (APTe) on the extraction yield of pectin was investigated by a software of Design Export 8.0.5b. The maximum extraction yield was obtained with the EWP of 20:1, EpH of 1.5, ETe of 90 掳C, ETi of 120 min, APpH of 3.0 and APTe of 50 掳C, which was consistent with the experimental value. We also found out that the pectin content decreased gradually during storage and sucrose concentration had a significant impact on the viscosity of pectin.