Barley malt wort is suited for the release of antifungal acids by LAB fermentation.
Higher wort extract contents led to more phenolic and organic acids released.
Phenolic acids released were far below the MIC against Fusarium culmorum.
Synergy between organic and phenolic acids was found at low pH.
Carboxylic acids by Lactobacillus brevis R2Δ accounted for the entire fungal inhibition.