Global redesign of a native β-barrel scaffold
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- 作者：Aaron J. Wolfea ; b ; Mohammad M. Mohammada ; Avinash K. Thakura ; b ; Liviu Movileanua ; b ; c ; lmovilea@syr.edu" class="auth_mail" title="E-mail the corresponding author ;
- 关键词：FhuA ; Single-molecule electrophysiology ; Ion channel ; Spontaneous gating ; Membrane protein engineering
- 刊名：Biochimica et Biophysica Acta (BBA)-Biomembranes
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：1858
- 期：1
- 页码：19-29
- 全文大小：1693 K
文摘
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Global engineering of ferric hydroxamate uptake component A was examined.
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Extensive charge neutralization of the β barrel maintained its stable structure.
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Spontaneous gating was modulated by interactions with an external polypeptide.
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Large extracellular loops of FhuA did not fold back into the pore lumen.
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This work demonstrated the versatility of a β-barrel scaffold to global engineering.