Improvement of the catalytic performance of a Bispora antennata cellulase by replacing the N-terminal semi-barrel structure
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- 作者：Fei Zhenga ; b ; Huoqing Huangb ; Xiaoyu Wanga ; b ; Tao Tub ; Qiong Liub ; Kun Mengb ; Yuan Wangb ; Xiaoyun Sub ; Xiangming Xiea ; Huiying Luob ; luohuiying@caas.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Cellulase ; Hybrid enzyme ; Glycosyl hydrolase (GH) family 5 ; Catalytic efficiency ; N-terminal (βα)-replacement
- 刊名：Bioresource Technology
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：218
- 期：Complete
- 页码：279-285
- 全文大小：1069 K
文摘
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An acidic mesophilic cellulase of GH5 (BaCel5) was identified in B. antennata.
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Effect of N-terminal barrel structures on its catalytic performance was studied.
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Replacing the N-terminal (βα)3 or (βα)4 barrel with that of TeEgl5A improved the specific activity up to 6.7-fold.
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Barrel structure replacement also improved the catalytic efficiency up to 4.7-fold.
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N-terminal replacement introduced more hydrogen bonds and enlarged catalytic cavity.