Alpha2A-adrenergic receptor and eNOS genetic polymorphisms are associated with exercise muscle vasodilatation in apparently healthy individuals

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• 作者：Rafael Amorim Belo Nunes ; ^{rafael.nunes@incor.usp.br} ; Lucia Pereira Barroso ; Alexandre da Costa Pereira ; Maria Urbana Pinto Brandã ; o Rondon ; Carlos Eduardo Negrã ; o ; José ; Eduardo Krieger ; Alfredo José ; Mansur
• 关键词：Muscle vasodilatation ; Forearm vascular conductance ; Exercise ; Genetic polymorphism ; Alpha-adrenergic receptor ; Endothelial nitric oxide synthase ; Bradykinin B2 receptor ; Cardiovascular genetics
• 刊名：IJC Heart & Vasculature
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：13
• 期：Complete
• 页码：14-18
• 全文大小：251 K
• 卷排序：13

文摘

Muscle vasodilatation during exercise has been associated with cardiovascular health and may be influenced by genetic variability. The purpose of this study was to evaluate functional genetic polymorphisms of physiologic pathways related to the regulation of the cardiovascular function (alpha-adrenergic receptors, endothelial nitric oxide synthase and bradykinin B2 receptor) and exercise muscle vasodilatation in apparently healthy men and women.MethodsWe enrolled 689 individuals without established cardiovascular disease that had attended a check-up program. The vasodilatation was studied with venous occlusion plethysmography and determined by the increase of vascular conductance during handgrip exercise. Genotypes for ADRA1A Arg347Cys (rs1048101), ADRA2A 1780 C > T (rs553668), ADRA2B Del 301–303 (rs28365031), eNOS 786 T > C (rs2070744), eNOS Glu298Asp (rs1799983) and BDKRB2 (rs5810761) polymorphisms were assessed by polymerase chain reaction followed by high resolution melting analysis.ResultsThe eNOS rs2070744 polymorphism was significantly associated with forearm vascular conductance during exercise in women. Women with CC genotype showed higher vasodilatation than carriers of TC and TT genotypes (p = 0.043). The ADRA2A rs553668 polymorphism was significantly associated with forearm vascular conductance during exercise in men. Men with TT genotype had higher vasodilatation than carriers of CT and CC genotypes (p = 0.025).ConclusionseNOS rs207074 polymorphism in women and ADRA2A rs553668 polymorphism in men were associated with the increase of forearm vascular conductance during handgrip exercise. These findings suggest that eNOS and ADRA2A genetic polymorphisms may be potential markers of exercise muscle vasodilatation.