Characterisation of lipase fatty acid selectivity using novel omega-3 pNP-acyl esters

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• 作者：Tim D. Nalder ; Susan Marshall ; Frederick M. Pfeffer ; Colin J. Barrow
• 关键词：p-Nitrophenyl ester ; EPA ; DHA ; Lipase ; Selectivity ; Steglich esterification
• 刊名：Journal of Functional Foods
• 出版年：January, 2014
• 年：2014
• 卷：6
• 期：Complete
• 页码：259-269
• 全文大小：785 K

文摘

Lipases have applications for the industrial processing of lipids, including concentrating and/or modifying fish oil derived omega-3 fatty acids, widely used as nutritional supplement and functional food ingredients. A range of para-nitrophenol (pNP) acyl esters were synthesised as a means to rapidly screen lipases for fatty acid selectivity using spectrophotometric detection. The chosen esters were based primarily on the most abundant fatty acids present in anchovy and tuna oils. pNP derivatives of C16:1 n-7, C18:1 n-9 (OA), C18:2 n-6 (LA), C18:3 n-3 (ALA), C20:5 n-3 (EPA) and C22:6 n-3 (DHA) were synthesised. Storage stability of these pNP derivatives was shown to be at least 6 months and all pNP derivatives, including those of EPA and DHA, were shown to be stable throughout the conditions of the assay. We applied the new assay substrates for the determination of fatty acid selectivity of five widely utilised lipases. Results showed that the lipase from Candida rugosa was the most selective in terms of omega-3 specificity, preferentially hydrolysing all other medium-long chain substrates. Lipases from Rhizomucor miehei and Thermomyces lanuginosa also showed selectivity, with a significant preference for saturated fatty acids. Candida antarctica lipase B and Aspergillus niger lipase were the least selective.