TAp63γ regulates hOGG1 and repair of oxidative damage in cancer cell lines

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• 作者：Sunil Upadhyay ; Aditi Chatterjee ; Barry Trink ; Matthias Sommer ; Edward Ratovitski ; David Sidransky
• 关键词：TAp63γ ; hOGG1 ; Transcription ; RNAi ; Oxidative damage
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2007
• 出版时间：18 May 2007
• 年：2007
• 卷：356
• 期：4
• 页码：823-828
• 全文大小：220 K

文摘

We showed that TAp63γ regulates hOGG1. Using chromatin immunoprecipitation (ChIP), we found that TAp63γ binds to the hOGG1 promoter. Reintroduction of wild-type TAp63γ into HEK 293 cells, induced transcription of hOGG1 promoter, leading to increase in RNA and protein. Using RNAi studies, we observed that TAp63γ-RNAi resulted in reduced hOGG1 RNA and protein in HeLa cells. This decrease in hOGG1 expression was associated with reduced cell viability upon oxidative damage. Taken together, our results indicate that hOGG1 is a direct target of TAp63γ, suggesting a role for TAp63γ in oxidative damage and repair.