- 作者：Arkady Uryash ; Heng Wu ; Jorge Bassuk ; Paul Kurlansky ; Jose A. Adams
- 关键词：Preconditioning ; Nitric oxide ; Myocardial infarction ; Endothelium-derived factors ; Second window of protection ; Cardioprotection ; Whole body periodic acceleration ; pGz
- 刊名：Life Sciences
- 出版年：2012
- 出版时间：4 September, 2012
- 年：2012
- 卷：91
- 期：5-6
- 页码：178-185
- 全文大小：905 K
Aims
Whole body periodic acceleration (pGz) is achieved with a motorized platform that moves the supine body with repetitive, sinusoidal head to foot motion. This increases pulsatile shear stress to the endothelium thereby activating endothelial nitric oxide synthase (eNOS) to produce increased release of nitric oxide (eNO). The current investigation was undertaken to determine whether or not pGz preconditioning can induce second window of protection (SWOP) cardioprotective affects in an in-vivo rat model of myocardial infarction.Main methods
Rats received 1, 3 or 7 daily pGz treatments of 60 minutes, or no pGz (CONT). Twenty-four hours after the last pGz treatment, ischemia reperfusion injury was produced by ligation of the left coronary artery (LCA) for 30 minutes followed by 90 minutes of reperfusion.
Key findings
All pGz preconditioned animals survived 90 minutes. Three and 7 days of pGz preconditioning reduced myocardial infarct size by 41 and 38 % respectively, with better left ventricular function. Additionally, pGz preconditioning increased eNOS, p-eNOS, Akt and p-Akt, HSP70 and nNOS proteins. L-NAME (NOS inhibitor) or Wortmannin (PI3/Akt inhibitor) 15 minutes prior to LCA ligation abolished pGz's cardioprotective effects.
Significance
pGz preconditioning provides SWOP, with increased survival, infarct size reduction, and improved contractility, in part due to up regulation of HSP70 and the PI3/Akt/eNOS , which last at least 72 hours.