Facile modulation of enantioselectivity of thermophilic Geobacillus zalihae lipase by regulating hydrophobicity of its Q114 oxyanion
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- 作者：Roswanira Abdul Wahaba ; roswanira@kimia.fs.utm.my" class="auth_mail" title="E-mail the corresponding author ; roswanira@kimia.fs.utm.my" class="auth_mail" title="E-mail the corresponding author ; Mahiran Basrib ; f ; mahiran@upm.edu.my" class="auth_mail" title="E-mail the corresponding author ; Raja Noor Zaliha Raja Abdul Rahmanc ; f ; g ; Abu Bakar Sallehd ; f ; g ; Mohd Basyaruddin Abdul Rahmanb ; f ; Thean Chor Leowe ; f ; g
- 关键词：T1 lipase ; Geobacillus zalihae ; 2DSN ; Site-directed mutagenesis ; Enantioselectivity ; Oxyanion
- 刊名：Enzyme and Microbial Technology
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：93-94
- 期：Complete
- 页码：174-181
- 全文大小：943 K
文摘
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Enantioselectivity of T1 altered after substitution of Glu114 with Leu and Met.
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Substitution with Met increased enantioselectivity of T1.
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Substitution with Leu reduced enantioselectivity of T1.
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Weakened electrostatic repulsions reduced enantioselectivity of Q114L.