The carbon starvation-inducible genes Rv2557 and Rv2558 of Mycobacterium tuberculosis are not required for long-term survival under carbon starvation and for virulence in SCID mice

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• 作者：Bhavna G. Gordhan ; Debbie A. Smith ; Bavesh D. Kana ; Gregory Bancroft ; Valerie Mizrahi
• 关键词：Mycobacterium ; Tuberculosis ; Carbon starvation ; Persistence
• 刊名：Tuberculosis
• 出版年：2006
• 出版时间：November 2006
• 年：2006
• 卷：86
• 期：6
• 页码：430-437
• 全文大小：205 K

文摘

Mycobacterium tuberculosis genes Rv2557 and Rv2558 have no known function. However, proteome, transcriptome and in situ hybridization studies have shown that these genes are significantly upregulated under carbon-starved conditions and in human granulomas, suggesting that they may play a role in persistence.

Single and double deletion mutants of M. tuberculosis H37Rv in Rv2557 and/or Rv2558 were generated to explore their individual and/or collective role(s) in growth and survival. The mutants were assessed for growth and survival in vitro under normal and nutrient-deprived conditions and for virulence in the SCID mouse model.

Although highly induced by carbon starvation, loss of Rv2557 and/or Rv2558 affected neither the long-term survival of M. tuberculosis under carbon-starved conditions in vitro, nor its virulence in SCID mice.