6,6鈥?Bieckol protects insulinoma cells against high glucose-induced glucotoxicity by reducing oxidative stress and apoptosis

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• 作者：Mi-Hwa Park^a ; Soo-Jin Heo^b ; Kil-Nam Kim^c ; Ginnae Ahn^d ; Pyo-Jam Park^e ; Sang-Ho Moon^f ; Byong-Tae Jeon^f ; Seung-Hong Lee^f ; ^{leesh80@kku.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：6 ; 6&prime ; -bieckol ; Rat insulinoma cells ; High glucose ; Oxidative stress ; Apoptosis
• 刊名：Fitoterapia
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：106
• 期：Complete
• 页码：135-140
• 全文大小：680 K

文摘

Pancreatic 尾 cells are highly sensitive to oxidative stress, which might play an important role in 尾 cell death in diabetes. The protective effect of 6,6′-bieckol, a phlorotannin polyphenol compound purified from Ecklonia cava, against high glucose-induced glucotoxicity was investigated in rat insulinoma cells. High glucose (30 mM) treatment induced the death of rat insulinoma cells, but treatment with 10 or 50 渭g/mL 6,6′-bieckol significantly inhibited the high glucose-induced glucotoxicity. Furthermore, treatment with 6,6′-bieckol dose-dependently reduced the level of thiobarbituric acid reactive substances, generation of intracellular reactive oxygen species, and the level of nitric oxide, all of which were increased by high glucose concentration. In addition, 6,6′-bieckol protected rat insulinoma cells from apoptosis under high-glucose conditions. These effects were associated with increased expression of the anti-apoptotic protein Bcl-2 and reduced expression of the pro-apoptotic protein Bax. These findings indicate that 6,6′-bieckol could be used as a potential nutraceutical agent offering protection against the glucotoxicity caused by hyperglycemia-induced oxidative stress associated with diabetes.