Molecular and biological characterization of highly infectious transcripts from full-length cDNA clones of broad bean wilt virus 1
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- 作者：Inmaculada Ferriola ; b ; 1 ; Silvia Ambró ; sa ; 1 ; 2 ; Dorivaldo M. da Silva Jr.c ; Bryce W. Falkb ; Luis Rubioa ; lrubio@ivia.es" class="auth_mail" title="E-mail the corresponding author
- 关键词：BBWV-1 ; Fabavirus ; Secoviridae ; Comovirinae ; Infectious clones
- 刊名：Virus Research
- 出版年：2016
- 出版时间：2 June 2016
- 年：2016
- 卷：217
- 期：Complete
- 页码：71-75
- 全文大小：1189 K
文摘
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Development of full-length cDNA clones of broad bean wilt virus 1 (BBWV-1).
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Transcripts of BBWV-1 full-length cDNA clones are highly infectious in different plant hosts.
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The biological and molecular characteristics of the viral transcripts were identical to the wild-type BBWV-1 isolate.
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The biological activity of viral transcripts was not affected by the addition of extra non-viral nucleotides at the 5′-terminus.
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The wide host range of this virus enables to studyhost-virus interactions and use it as viral vector for many plant species.