Dual targeted poplar ferredoxin NADP+ oxidoreductase interacts with hemoglobin 1
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- 作者：Soile Jokipii-Lukkaria ; Alexander J. Kastaniotisb ; Vimal Parkashc ; Robin Sundströ ; mc ; Né ; lida Leiva-Erikssond ; Yvonne Nymalmc ; Olga Blokhinae ; Eija Kukkolae ; Kurt V. Fagerstedte ; Tiina A. Salminenc ; Esa Lä ; ä ; rä ; f ; Leif Bü ; lowd ; Steffen Ohlmeierb ; J. Kalervo Hiltunenb ; Pauli T. Kalliog ; Hely Hä ; ggmana ; hely.haggman@oulu.fi" class="auth_mail" title="E-mail the corresponding author
- 关键词：BCIP/NPT ; 5-bromo-4-chloro-3-indolylphosphate/nitroblue tetrazolium ; BiFC ; bimolecular fluorescence complementation ; 2-DE ; two-dimensional gel electrophoresis ; DETA NONOate ; (Z)-1-[N-(2-aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-ium-1 ; 2-diolate ; DIG ; digoxigenin ; Hbs ; hemoglobins ; hFNR ; heterotrophic ferredoxin NADP+ oxidoreductase ; MS ; mass spectrometry ; NO ; nitric oxide ; NOD ; NO dioxygenation ; nsHb ; non-symbiotic hemoglobin ; ORF ; open reading frame ; PIPES ; piparazine-N ; N&rsquo ; -bis(ethanesul
- 刊名：Plant Science
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：247
- 期：Complete
- 页码：138-149
- 全文大小：2366 K
文摘
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A novel cytosolic root-type ferredoxin NADP+ oxidoreductase (hFNR) was found.
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HFNR was able to interact with Hb1 and relieve NO toxicity in living cells.
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The coexpression of hFNR and truncated Hb did not affect NO sensitivity of cells.
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This is a first report on dual targeting of plant hFNR to both plastids and cytosol.
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The result may help to explain the protein dual targeting phenomenon.