Rapid isolation of pure Complement Factor H from serum for functional studies by the use of a monoclonal antibody that discriminates FH from all the other isoforms
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- 作者：Silvia Berra ; silvia.berra@unimi.it" class="auth_mail" title="E-mail the corresponding author ; silvia.berra@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Alberto Clivio
- 关键词：AP ; alternative pathway ; aHUS ; atypical haemolytic uraemic syndrome ; AMD ; age-related macular degeneration ; BCIP ; 5-bromo-4-chloro-3-indolyl-phosphate ; CCP ; complement control protein ; CFH ; Complement Factor H gene ; DAA ; decay acceleration activity ; FB ; complement factor B ; FH ; Complement Factor H ; FHL-1 ; factor H&ndash ; like protein 1 ; FHR ; factor H&ndash ; related ; FI ; complement factor I ; GAGs ; glycosaminoglycans ; IP ; immunoprecipitation ; NBT ; nitro-blue tetrazolium ; MPGN ; Membranoproliferative
- 刊名：Molecular Immunology
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：72
- 期：Complete
- 页码：65-73
- 全文大小：1240 K
文摘
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A monoclonal antibody (5H5) specific for Complement Factor H but unreactive with FHL-1 and FHR-proteins is described.
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5H5 recognises both the uncleaved and the cleaved form of FH, useful for quality control.
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A fast and specific purification method for Complement Factor H is proposed.
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Two functional assays to be used in conjunction with the purification method are proposed.
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A specific quantitative assay using mAb 5H5 was developed.