Quantifying autophagy: Measuring LC3 puncta and autolysosome formation in cells using multispectral imaging flow cytometry
详细信息 查看全文
- 作者：Haley R. Pugsley haley.pugsley@emdmillipore.com
- 关键词：MIFC ; multispectral imaging flow cytometry ; CQ ; chloroquine ; BDI ; Bright Detail Intensity ; BDS ; Bright Detail Similarity ; LC3 ; microtubule associated protein 1A/1B-light chain 3 ; BF ; brightfield ; AF647 ; Alexa Fluor® ; 647 ; Rd ; Fisher&rsquo ; s discriminant ratio ; HBSS ; Hank&rsquo ; s Balanced Salt Solution ; EBSS ; Earle&rsquo ; s Balanced Salt Solution
- 刊名：Methods
- 出版年：2017
- 出版时间：1 January 2017
- 年：2017
- 卷：112
- 期：Complete
- 页码：147-156
- 全文大小：1701 K
- 卷排序：112
文摘
A review of the predominant methods to measure autophagy via MIFC. Bright Detail Intensity and Spot Count work for measuring LC3 puncta accumulation. Bright Detail Similarity (BDS) can measure the formation of the autolysosome. Spot Count vs BDS provides the most comprehensive information on autophagy. There is not a single correct method for measuring autophagy using MIFC.