Bead-beating artefacts in the Bacteroidetes to Firmicutes ratio of the human stool metagenome
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- 作者：Heidi C. Vebø ; a ; 1 ; Magdalena Kauczynska Karlssona ; Ekaterina Avershinab ; Lene Finnbya ; Knut Rudib ; knut.rudi@nmbu.no" class="auth_mail" title="E-mail the corresponding author
- 关键词：OTU ; Operational Taxonomic Unit
- 刊名：Journal of Microbiological Methods
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：129
- 期：Complete
- 页码：78-80
- 全文大小：305 K
文摘
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Bead-beating has a major impact on the determined composition of the human stool microbiota.
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Different bead-beating instruments from the same producer gave a 3-fold difference in the Bacteroidetes to Firmicutes ratio.
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Proper harmonization and optimisation of bead-beating are needed to avoid severe artefacts in the literature.