A comparison of different pre-lysis methods and extraction kits for recovery of Streptococcus agalacticae (Lancefield group B Streptococcus) DNA from whole blood
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- 作者：Rachael M. Burkea ; b ; James P. McKennab ; Ciara Coxb ; Peter V. Coyleb ; Michael D. Shieldsa ; c ; Derek J. Fairleya ; b ; derek.fairley@belfasttrust.hscni.net" class="auth_mail" title="E-mail the corresponding author
- 关键词：Group B Streptococcus ; Extraction ; Achromopeptidase
- 刊名：Journal of Microbiological Methods
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：129
- 期：Complete
- 页码：103-108
- 全文大小：311 K
文摘
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Yields of genomic DNA from GBS are exceptionally low (< 2%) using conventional extraction.
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Mechanical pre-lysis (bead-beating) improved yield by over 50-fold.
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Enzymatic pre-lysis (achromopeptidase) was also found to be very effective.
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A rapid (5 min) room-temperature achromopeptidase lysis protocol is presented.