- 作者：Karen Tan ; karen.tan@mohh.com.sg ; Lizhen Ong ; Sunil K. Sethi ; Sharon Saw
- 关键词：PTH ; (parathyroid hormone) ; CKD ; (chronic kidney disease) ; iPTH ; (intact PTH) ; ESRD ; (end stage renal disease) ; CV ; (coefficient of variation) ; (ECLIA) ; electro-chemiluminescence immunoassay ; (ALP) ; alkaline phosphatase ; (Hb) ; Hemoglobin ; (HbA1c) ; hemoglo
- 刊名：Clinical Biochemistry
- 出版年：2013
- 出版时间：June, 2013
- 年：2013
- 卷：46
- 期：9
- 页码：781-786
- 全文大小：625 K
Objectives
The COBAS Elecsys PTH(1-84) assay is a novel, electro-chemiluminescence immunoassay that exclusively measures full-length parathyroid hormone (PTH). The aim of this study is to compare the automated biointact Elecsys PTH(1-84) assay with four contemporary, iPTH assays in chronic kidney disease (CKD) patients.Design and methods
We compared the Elecsys PTH(1-84) assay with four iPTH assays (Siemens ADVIA Centaur, Ortho Clinical Diagnostics (OCD) VITROS, Beckman Access2, Abbott ARCHITECT) in the measurement of PTH in 83 local CKD patients. Majority of the patients (44) had CKD but were not on dialysis, 15 were on hemodialysis, 15 were on peritoneal dialysis, and 9 were post-renal transplant. The precision performance and correlation of the assays were determined. PTH(1-84) concentrations were correlated with calcium, phosphate, alkaline phosphatase, hemoglobin, HbA1c and lipid concentrations.
Results
The Elecsys PTH(1-84) assay showed comparable precision and good correlation with the iPTH assays. Although the four different iPTH assays correlated well with each other, there was significant discrepancy among assays. The discrepancy among assays increased with increasing PTH concentrations. The ADVIA Centaur and ARCHITECT assays measured significantly higher PTH concentrations than the VITROS and Access2 assays. PTH(1-84) showed a positive association with phosphate and alkaline phosphatase and an inverse association with HbA1c. There was no significant association with lipid concentrations.
Conclusions
The third generation Elecsys PTH(1-84) assay had comparable precision performance and correlated well with second generation iPTH assays. However, significant discrepancy was found among the four iPTH assays in measuring iPTH in CKD patients.