Dietary probiotic Bacillus

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• 作者：Jiqiu Li ; Beiping Tan ; Kangsen Mai
• 关键词：Bacillus OJ ; Isomaltooligosaccharides ; Litopenaeus vannamei ; Immunity ; Microbial populations ; Disease resistance ; Probiotics
• 刊名：Aquaculture
• 出版年：2009
• 出版时间：3 June 2009
• 年：2009
• 卷：291
• 期：1-2
• 页码：35-40
• 全文大小：588 K

文摘

In order to reveal the potential of Bacillus OJ (PB) as probiotic and the synergistic effects of PB and isomaltooligosaccharides on intestine microbial populations, immune responses and disease resistance of shrimp (Litopenaeus vannamei), a 28-day feeding experiment was conducted in shrimp by feeding PB at doses of 0, 10⁸, and 10¹⁰ CFU/g feed with or without 0.2 % isomaltooligosaccharides in shrimp diets. After a 14-day feeding experimental period, six shrimp per tank were sampled for bacterial quantification and immunity determination. Then all the shrimp left were challenged by white spot syndrome virus. Results showed that, with the increasing doses of PB in diets, shrimp survivals and immune parameters generally increased whereas the counts of total viable bacteria and Vibrio decreased. Shrimp fed with PB at dose of 10¹⁰ CFU/g of feed produced significantly higher survivals and immune parameters than the control groups. Although the effect of 0.2 % isomaltooligosaccharides alone was negligible, 0.2 % isomaltooligosaccharides in combination with 10⁸ CFU/g feed PB produced significantly positive synergistic effects on shrimp immune responses and disease resistance. While the doses of PB increased to 10¹⁰ CFU/g feed, the synergistic effects significantly decreased. Then, our results confirm the potential of PB as dietary probiotic and the synergistic effects of PB and isomaltooligosaccharides in shrimp.