Use of DArT markers as a means of better management of the diversity of olive cultivars
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- 作者：Sergio G. Atienza ; Ra煤l de la Rosa ; Mar铆a C. Dom铆nguez-Garc铆a ; Antonio Mart铆n ; Andrzej Kilian ; Angjelina Belaj
- 关键词：Olea europaea L. ; Germplasm collections ; Genetic resources ; Genetic relationships
- 刊名：Food Research International
- 出版年：December, 2013
- 年：2013
- 卷：54
- 期：2
- 页码：2045-2053
- 全文大小：726 K
文摘
DArT (Diversity Array Technology) markers assessed in a large set of olive cultivars have been used to evaluate their utility for the management of the World Olive Germplasm Bank (WOGB鈥擟贸rdoba) of IFAPA Centre 鈥淎lameda del Obispo鈥?C贸rdoba (Spain). The coefficient of similarity of Kulczynsky (K1), which calculates the ratio between common markers relative to the number of unique markers (range 0 to 鈭?, was used for such purpose. Low K1 values (lower than 3.61) were obtained for the majority of the pair of cultivars (99.80% of the total). Low and similar ranges of K1 values were also found in both a seedling progeny of 鈥楶icual鈥?#xA0;脳 鈥楢rbequina鈥?and a set of wild olives included in the study. These results may suggest that most of the WOGB鈥擟贸rdoba cultivars studied probably were derived from outcrossing events. As expected, low similarity values were found among different cultivars with the same naming (homonyms). On the contrary, a total of 105 pairs (0.20%) showed K1 values above 14.58, implying thus low genetic differentiation. These high K1 values suggest the presence of potential redundant germplasm in the collection which likely includes synonyms, accumulation of somatic mutations, redundancies from prospecting surveys, and planting errors. Thus, the genetic relationships revealed by DArT markers among cultivars are of great interest for management of olive collections and could be useful to increase the performance of future breeding programs by optimizing the choice of parents in combination with phenotypic information. Finally, the transferability of DArT markers to wild olives has been confirmed.