The macrophage migration inhibitory factor ? 173 G/C polymorphism is not significantly associated with necrotizing enterocolitis in preterm infants

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• 作者：Giusi Prencipe ; Cinzia Auriti ; Rita Inglese ; Giulia Gallusi ; Andrea Dotta ; Fabrizio De Benedetti
• 关键词：Cytokines ; Innate immunity ; MIF ; NEC
• 刊名：Journal of Pediatric Surgery
• 出版年：2013
• 出版时间：July, 2013
• 年：2013
• 卷：48
• 期：7
• 页码：1499-1502
• 全文大小：115 K

文摘

Background and Purpose

Necrotizing enterocolitis (NEC) is a leading cause of morbidity and mortality among premature infants. Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine that has been implicated in the pathophysiology of inflammatory bowel diseases. The MIF promoter contains a functionally relevant single nucleotide polymorphism (SNP) G¡úC at position ? 173, with the MIF ? 173*C allele being associated with higher MIF expression in vitro and with higher MIF levels in vivo. The aim of this study was to investigate whether the G/C polymorphism at ? 173 of the MIF promoter is associated with the development of NEC.

Methods

In this retrospective cohort study, 107 preterm infants (GA ¡Ü 32 weeks), of whom 41 had NEC (NEC Stage I n = 20, Stage II n = 3, Stage III n = 18) and 66 were not affected, were genotyped for the MIF ? 173 SNP. MIF genotyping was carried out by PCR and DHPLC.

Results

We did not find significant differences in the prevalence of the ? 173 G/C polymorphism and in the distribution of the ? 173 MIF genotype in infants with NEC compared to controls. Moreover, we did not observe an association between the polymorphism and mortality.

Conclusions

The polymorphism ? 173 G/C of the MIF promoter does not appear to be of major importance in the pathophysiology of NEC in preterm infants.