Time-resolved NMR metabolomics of plant cells based on a microfluidic chip

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• 作者：Jan Maisch^a ; ^{Jan.Maisch@kit.edu" class="auth_mail" title="E-mail the corresponding author} ; Kristina Kreppenhofer^b ; Silke Bü ; chler^c ; ^d ; Christian Merle^c ; ^d ; Shukhrat Sobich^b ; Benjamin Gö ; rling^c ; ^d ; Burkhard Luy^c ; ^d ; Ralf Ahrens^b ; Andreas E. Guber^b ; Peter Nick^a
• 关键词：Microfluidics ; NMR metabolomics ; Tobacco cells (Nicotiana tabacum L. cv. BY-2)
• 刊名：Journal of Plant Physiology
• 出版年：2016
• 出版时间：1 August 2016
• 年：2016
• 卷：200
• 期：Complete
• 页码：28-34
• 全文大小：1005 K

文摘

The plant secondary metabolism generates numerous compounds harbouring pharmaceutical activity. In plants, these compounds are typically formed by different and specialised cell types that have to interact constituting a metabolic process chain. This interactivity impedes biotechnological production of secondary compounds, because cell differentiation is suppressed under the conditions of a batch bio-fermenter. We present a novel strategy to address this limitation using a biomimetic approach, where we simulate the situation in a real tissue by a microfluidic chamber system, where plant cells can be integrated into a process flow. We show that walled cells of the plant model tobacco BY-2 can be successfully cultivated in this system and that physiological parameters (such as cell viability, mitotic index and division synchrony) can be preserved over several days. The microfluidic design allows to resolve dynamic changes of specific metabolites over different stages of culture development. These results serve as proof-of-principle that a microfluidic organisation of cultivated plant cells can mimic the metabolic flows in a real plant tissue.