Differential modulation of the chaperone-like activity of HSP-1/2, a major protein of horse seminal plasma by anionic and cationic surfactants
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- 作者：C. Sudheer Kumar ; Musti J. Swamy ; mjswamy@uohyd.ac.in ; mjswamy1@gmail.com ;
- 关键词：ADH ; alcohol dehydrogenase ; Ala-16 ; alanine palmityl ester hydrochloride ; ANS ; 8-anilinonaphthalene-1-sulphonate ; CD ; circular dichroism ; CLA ; chaperone-like activity ; CTAB ; cetyl trimethylammonium bromide ; FnII ; fibronectin type-II ; HSP-1/2 ; horse seminal plasma proteins 1 and 2 ; SDS ; sodium dodecyl sulphate ; SDBS ; sodium dodecyl benzenesulphonate ; TBS ; 50 ; mM tris buffer containing 0.15 ; M NaCl ; 5 ; mM EDTA ; pH 7.4
- 刊名：International Journal of Biological Macromolecules
- 出版年：2017
- 出版时间：March 2017
- 年：2017
- 卷：96
- 期：Complete
- 页码：524-531
- 全文大小：901 K
- 卷排序：96
文摘
Anionic and neutral surfactants inhibit thermal aggregation of ADH. Cationic surfactants induce aggregation of ADH even at room temperature. Anionic surfactants increase the CLA of HSP-1/2 whereas cationic surfactants decrease it. The above differences are explained based on electrostatic interactions.