The in vitro

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• 作者：A.B. Essop ; R.L. van Zyl ; S.F. van Vuuren ; D. Mulholl ; A.M. Viljoen
• 关键词：Anti-inflammatory ; Antimicrobial ; Anti-oxidant ; Cytotoxicity ; Hermannia
• 刊名：Journal of Ethnopharmacology
• 出版年：2008
• 出版时间：28 October 2008
• 年：2008
• 卷：119
• 期：3
• 页码：615-619
• 全文大小：519 K

文摘

Hermannia species are widely used in traditional medicine in southern Africa, however no extensive study has been conducted on this genus. The acetone extracts of 12 indigenous Hermannia species (flowers, stems and leaves combined) were evaluated for various pharmacological activities. All investigated species displayed promising antimicrobial activity, with Hermannia saccifera being the most active against Staphylococcus aureus (MIC = 19.5 μg/ml), Bacillus cereus (MIC = 19.5 μg/ml) and Enterococcus faecalis (MIC = 125 μg/ml). Time-kill studies on H. saccifera against S. aureus indicated bacteriostatic activity at 1.25, 2.5 and 5.0 % , and a concentration of 7.5 % achieved complete bactericidal activity after 4 h. Ten of the 12 species indicated good free radical scavenging activity, with H. cuneifolia demonstrating the most promising activity in the 2,2-diphenyl-1-picrylhydrazyl (DPPH:IC₅₀ = 10.26 μg/ml) and 2,2′-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS⁺/TEAC: IC₅₀ = 10.32 μg/ml) assays. In addition, all species exhibited moderate anti-inflammatory activity in the 5-lipoxygenase assay with the exception of H. cuneifolia (IC₅₀ = 15.32 μg/ml). Overall, the selected species were low in cytotoxicity, except for H. saccifera and H. trifurca. Several Hermannia species indicated promising in vitro biological activity which relate to their traditional use in treating a number of disease states.