Characterization of a novel esterase Rv1497 of Mycobacterium tuberculosisH37Rv demonstrating β-lactamase activity
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- 作者：Gurpreet Singha ; Arbind Kumara ; Stuti Aryaa ; Umesh Dutt Guptab ; Kashmir Singha ; Jagdeep Kaura ; jagsekhon@yahoo.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Esterase ; β-Lactamase ; Mycobacterium ; Rv1497
- 刊名：Enzyme and Microbial Technology
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：82
- 期：Complete
- 页码：180-190
- 全文大小：3356 K
文摘
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Rv1497 of M. tuberculosis H37Rv was predicted to be involved in lipid metabolism.
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Rv1497 demonstrated both esterase and β-lactamase activities.
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Serine within ‘SXXK’ motif was identified as catalytic residue in enzymatic activities.
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The catalytic residues of Rv1497 were determined to be Ser88, Tyr-175 and His355.
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The expression of Rv1497 gene was significantly up-regulated during acidic stress.