Stark fluorescence spectroscopy reveals two emitting sites in the dissipative state of FCP antennas
详细信息 查看全文
- 作者：Md. Wahadoszamen ; Artur Ghazaryan ; H ; e E. Cingil ; Anjue Mane Ara ; Claudia B眉chel ; Rienk van Grondelle ; Rudi Berera
- 关键词：Diatoms ; Light harvesting antenna ; Energy dissipation ; Nonphotochemical quenching ; Charge transfer states ; Stark spectroscopy
- 刊名：Biochimica et Biophysica Acta (BBA)/Bioenergetics
- 出版年：January, 2014
- 年：2014
- 卷：1837
- 期：1
- 页码：193-200
- 全文大小：791 K
文摘
Diatoms are characterized by very efficient photoprotective mechanisms where the excess energy is dissipated as heat in the main antenna system constituted by fucoxanthin-chlorophyll (Chl) protein complexes (FCPs). We performed Stark fluorescence spectroscopy on FCPs in their light-harvesting and energy dissipating states. Our results show that two distinct emitting bands are created upon induction of energy dissipation in FCPa and possibly in FCPb. More specifically one band is characterized by broad red shifted emission above 700 nm and bears strong similarity with a red shifted band that we detected in the dissipative state of the major light-harvesting complex II (LHCII) of plants . We discuss the results in the light of different mechanisms proposed to be responsible for photosynthetic photoprotection.