High pressure/temperature treatments to inactivate highly infectious prion subpopulations

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• 作者：Philipp Heindl ; Avelina Fern ; ez Garcia ; Peter Butz ; Bernhard Trierweiler ; Heiner Voigt ; Eberhard Pfaff ; Bernhard Tauscher
• 关键词：High pressure ; 263K strain ; Scrapie prions ; Inactivation kinetics ; Enzyme immunoassay ; EIA ; Prion infectivity ; Prion inactivation
• 刊名：Innovative Food Science and Emerging Technologies
• 出版年：2008
• 出版时间：July 2008
• 年：2008
• 卷：9
• 期：3
• 页码：290-297
• 全文大小：517 K

文摘

High hydrostatic pressure can be used for gentle pasteurization of food as well as a physical parameter to study the stability and energetics of biomolecules. High pressure has been recently postulated as a feasible technology to decontaminate scrapie infectious materials. Here we discuss the kinetic parameters driving the inactivation of the Transmissible Spongiform Encephalopathy agents and the perspectives of pressure as a thermodynamic parameter to obtain a deeper insight into the aggregation of the 263K strain of scrapie. At 60–80 °C an efficient pressure inactivation of infectious scrapie prions was observed during short pressure treatments at 800 MPa (3 × 5 min cycles). However, discrepancies between in vivo infectivity counts and the results of an enzyme immunoassay further revealed that the infectivity was inactivated faster and much more efficiently than PrP^res was degraded, indicating that pressure affects a highly infectious subpopulation of scrapie prions.