Determination of the oxidative burst chemiluminescent response of avian and murine-derived macrophages versus corresponding cell lines in relation to stimulation with Salmonella serotypes
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- 作者：Chadfield ; Mark ; Olsen ; John
- 关键词：AUC ; area under curve ; CL ; chemiluminescent ; DMEM ; Dulbecco’ ; s modified eagles medium (cell culture medium) ; fMLP ; N-formyl-methionyl-leucyl-phenylalanine ; HBSS ; Hanks balanced salt solution ; LB ; Luria Bertani (agar or broth) ; pkH ; maximum peak height
- 刊名：Veterinary Immunology and Immunopathology
- 出版年：2001
- 出版时间：August 10, 2001
- 年：2001
- 卷：80
- 期：3-4
- 页码：289-308
- 全文大小：248 K
文摘
In contrast to mammalian systems, avian species lack a resident or harvestable macrophage population in the abdominal exudate. Peritoneal macrophages in the chicken can be elicited if an inflammatory agent such as sephadex is injected. This study examines the kinetics of different macrophage populations, derived by different methods of isolation and from different hosts, with respect to the elicited oxidative burst upon infection with host-adapted Salmonella serotypes.