Ecological roles and release patterns of acylated homoserine lactones in Pseudomonas sp. HF-1 and their implications in bacterial bioaugmentation

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• 作者：Mei-zhen Wang^a ; ^b ; Xin Zheng^a ; Hong-zhen He^a ; Dong-sheng Shen^a ; ^b ; Hua-jun Feng^a ; ^b ; ^{fenghuajun@mail.zjgsu.edu.cn}
• 关键词：AHLs ; acylated homoserine lactones ; C6-HSL ; N-hexanoyl-homoserine lactone ; EPS ; extracellular polymeric substances ; ¦Â-Gal ; ¦Â-Galactosidase ; HPLC ; high-performance liquid chromatography ; ISM ; inorganic salt medium ; LB ; Luria&ndash ; Bertani ; 3-oxo-C6-HSL
• 刊名：Bioresource Technology
• 出版年：2012
• 出版时间：December, 2012
• 年：2012
• 卷：125
• 期：Complete
• 页码：119-126
• 全文大小：342 K

文摘

To enable development of a better bacterial bioaugmentation system for tobacco wastewater treatment, the roles and release patterns of acylated homoserine lactones (AHLs) in Pseudomonas sp. HF-1 were evaluated. Swarming was found to be induced by N-hexanoyl-homoserine lactone (C₆-HSL) and N-3-oxo-hexanoyl-homoserine lactone (3-oxo-C₆-HSL); the formation of extracellular polymeric substances (EPS) was induced by 3-oxo-C₆-HSL, C₆-HSL and N-3-oxo-octanoyl-homoserine lactone (3-oxo-C₈-HSL); and biofilm formation was induced by C₆-HSL and 3-oxo-C₈-HSL. When the culture conditions were 25 ¡ãC, pH 5-6, 3 % inoculum, 1.5 g L^?1 nicotine and 1 % NaCl, the amount of AHLs released was sufficient for quorum sensing of swarming and EPS formation for strain HF-1, which was beneficial to the startup stage during bioaugmentation. When strain HF-1 was cultured at pH 8 in the presence of 1.2-1.8 g L^?1 of nicotine and 1 % NaCl, the threshold for quorum sensing of biofilm formation was reached and the bioaugmentation system showed an efficient performance.