Characterization of ribonuclease III from Brucella
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- 作者：Chang-Xian Wua ; 1 ; Xian-Jin Xua ; 1 ; Ke Zhenga ; Fang Liua ; Xu-Dong Yanga ; Chuang-Fu Chenb ; Huan-Chun Chena ; Zheng-Fei Liua ; lzf6789@mail.hzau.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：APS ; ammonium persulphate ; bp ; base pair ; dsRBD ; dsRNA-binding domain ; dsRNA ; double strand RNA ; EMSA ; electrophoretic mobility shirt assay ; IPTG ; isopropyl-D-thiogalactoside ; LB ; Luria-Bertani medium ; PCR ; polymerase chain reaction ; PH ; hydrogen ion concentration ; RIIID ; RNase III domain ; r/min ; rotation per minute ; SDS ; sodium dodecyl sulfonate
- 刊名：Gene
- 出版年：2016
- 出版时间：1 April 2016
- 年：2016
- 卷：579
- 期：2
- 页码：183-192
- 全文大小：1610 K
文摘
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Brucella RNase III can bind sRNA and pre-miRNA.
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Bm-RNase III cleavage is bivalent metal cations- and alkaline buffer-dependent.
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The 133th Glutamic acid residue was required for catalytic activity of Bm-RNase III.
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Brucella RNase III was expressed differently in virulent and vaccine strains.