Single-image based methods used for non-invasive volume estimation of cancer spheroids: a practical assessing approach based on entry-level equipment
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- 作者：Filippo Piccininia ; Anna Teseib ; Alessandro Bevilacquaa ; c ; alessandro.bevilacqua@unibo.it" class="auth_mail" title="E-mail the corresponding author
- 关键词：Widefield microscopy ; Oncology ; Pre-clinical studies ; 3D cell aggregates ; Volume estimation ; Image processing
- 刊名：Computer Methods and Programs in Biomedicine
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：135
- 期：Complete
- 页码：51-60
- 全文大小：2024 K
文摘
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Volume is one of the most important features to characterise a tumour.
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Multicellular spheroids are commonly used as 3D tumour models.
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We revised the exiting single-image based volume assessment methods for spheroids.
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We validated ReViSP using a light-sheet fluorescent microscope, and upgraded it.
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We presented a novel approach to assess effectiveness of volume estimation method.